公开(公告)号：US06204040B1

公开(公告)日：2001-03-20

申请号：US09296284

申请日：1999-04-22

Applicant: Eui-Sung Choi ,  Sang-Ki Rhee ,  Eun-Hae Lee

Inventor： Eui-Sung Choi ,  Sang-Ki Rhee ,  Eun-Hae Lee

IPC: C12N904

CPC classification number: C12N9/0006 ,  C12P7/60

Abstract: The invention relates to the fields of molecular biology, bacteriology and industrial fermentation. More specifically, the invention provides isolated nucleic acid molecules encoding the three subunits of a novel, membrane-bound, Gluconobacter oxydans sorbitol dehydrogenase (SDH) of the invention and vectors and host cells containing said isolated nucleic acid molecules. The invention further provides isolated polypeptides for the three subunits ofthe SDH enzyme of the invention, and processes for the production of L-sorbose and 2 keto-L-gulonic acid.

Abstract translation: 本发明涉及分子生物学，细菌学和工业发酵领域。 更具体地，本发明提供了编码本发明的新颖的，膜结合的氧化葡糖酸葡萄球菌山梨糖醇脱氢酶（SDH）的三个亚基的分离的核酸分子和含有所述分离的核酸分子的载体和宿主细胞。 本发明还提供了本发明的SDH酶的三个亚基的分离的多肽，以及L-山梨糖和2-酮-L-古洛糖酸的生产方法。