公开(公告)号：US11202421B2

公开(公告)日：2021-12-21

申请号：US16361451

申请日：2019-03-22

申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

发明人： Vincent Laurens Adrianus Kock ,  Johannes Geert Jan Feitsma ,  Raoul Jacobus Johannes Maria Frijters

IPC分类号： A01H5/12 ,  A01H6/02 ,  C12N15/82 ,  C12Q1/6895

摘要： The present invention relates to an allele designated alpha-WOLF 8 which confers resistance to at least one Peronospora farinosa f. Sp. spinacea race, wherein the protein encoded by said allele is a CC-NB S-LRR protein that comprises in its amino acid sequence: a) the motif “MAEIGYSVC” (SEQ ID NO: 15) at its N-terminus; and b) the motif “KWMCLR” (SEQ ID NO: 16); and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 12. When the allele is homozygously present in a spinach plant confers complete resistance to Peronospora farinosa f. Sp. spinacea races pfs:1, pfs:2, pfs:6, pfs:8 and pfs:15, and isolate US1508, and confers intermediate resistance to pfs:5, pfs:10 and pfs:16, and does not confer resistance to pfs:3, pfs:4, pfs:7, pfs:9, pfs:11, pfs:12, pfs:13 and pfs:14.

公开(公告)号：US20190241905A1

公开(公告)日：2019-08-08

申请号：US16361564

申请日：2019-03-22

申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.

发明人： Vincent Laurens Adrianus Kock ,  Johannes Geert Jan Feitsma ,  Raoul Jacobus Johannes Maria Frijters

IPC分类号： C12N15/82 ,  C12Q1/6895

CPC分类号： C12N15/8282 ,  A01H5/12 ,  C12Q1/6895 ,  C12Q2600/13

摘要： The present invention relates to a method for modifying the resistance profile of a spinach plant to Peronospora farinosa f sp. spinaciae, comprising introducing a WOLF allele or a resistance-conferring part thereof into the genome of said spinach plant, or modifying an endogenous WOLF allele in the genome of said spinach plant. A WOLF allele encodes a CC-NBS-LRR protein that comprises in its amino acid sequence: the motif “MAEIGYSVC” at its N-terminus, and the motif “KWMCLR” for alpha-type WOLF proteins or “HVGCVVDR” for beta-type WOLF proteins. The invention relates to the WOLF alleles referred to in Table 3. The invention further provides a method for selecting a spinach plant comprising a novel WOLF gene that confers resistance to Peronospora farinosa f. sp. spinaciae in a spinach plant and a method for identifying a WOLF allele that confers resistance to one or more pathogenic races of Peronospora farinosa f sp. spinaciae in a spinach plant and to primers for use in these methods.