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公开(公告)号：US11345925B2
公开(公告)日：2022-05-31
申请号：US16342143
申请日：2017-10-13
发明人： Jennifer Kara Barry , James J English , Kevin A Fengler , Zhenglin Hou , Lu Liu , Eric Schepers , Jeffrey Sopa , Ingrid Udranszky
IPC分类号： C12N15/82 , C07K14/415 , A01N65/04
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US10227608B2
公开(公告)日：2019-03-12
申请号：US15116740
申请日：2015-02-06
发明人： Jennifer Barry , Ryan Michael Gerber , Lu Liu , Amy Lum , Eric Schepers , Nasser Yalpani , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US20180094278A1
公开(公告)日：2018-04-05
申请号：US15840073
申请日：2017-12-13
发明人： Andre R Abad , Zhenglin Hou , Lu Liu , Brad Poland , Ute Schellenberger , Ingrid Udranszky , Jimei Wang , Jun-Zhi Wei , Thomas C Wolfe , Weiping Xie , Lan Zhou , Genhai Zhu
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are isolated insecticidal proteins and nucleic acids. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
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24.发明授权Compositions and methods comprising sequences having meganuclease activity 有权包含具有大范围核酸酶活性的序列的组合物和方法公开(公告)号：US09499827B2
公开(公告)日：2016-11-22
申请号：US13886317
申请日：2013-05-03
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-Bin Liu , Azalea Ong , Sergei Svitashev , Joshua K Young
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
摘要翻译： 提供了包含具有大范围核酸酶活性的多核苷酸和多肽的组合物和方法。 还提供了具有大范围核酸酶序列的核酸构建体，酵母，植物，植物细胞，外植体，种子和颗粒。 提供了使用大范围核酸酶序列的各种方法。 这样的方法包括例如用于生产在宽温度范围内具有增加的活性的大范围核酸酶的方法，用于生产酵母，植物，植物细胞，外植体或包含具有增加的活性的大范围核酸酶的种子的方法。
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公开(公告)号：US09475847B2
公开(公告)日：2016-10-25
申请号：US13800233
申请日：2013-03-13
发明人： Daniel J. Altier , Jennifer K. Barry , Carol A. Hendrick , Lu Liu , Phillip A. Patten , Claudia D. Perez-Ortega , Eric J. Schepers , Weiping Xie , Nasser Yalpani , Jianzhou Zhao , Xiaohong Zhong , Genhai Zhu
IPC分类号： C07K14/225 , A01N37/46 , C07K14/195 , A01N63/02 , C12N15/82
CPC分类号： A01N37/46 , A01N63/02 , C07K14/195 , C07K14/225 , C07K2319/00 , C07K2319/21 , C12N15/8205 , C12N15/8207 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译： 提供了控制害虫的组成和方法。 该方法涉及用编码杀虫蛋白的核酸序列转化生物体。 特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。 因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。 组合物是杀虫的核酸和细菌物种的蛋白质。 这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，包括植物，作为分离其他同源（或部分同源的）基因的探针。 杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群并生产具有杀虫活性的组合物。
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26.发明申请NOVEL BACILLUS THURINGIENSIS GENE WITH COLEOPTERAN ACTIVITY 审中-公开新颖的BACILLUS THURINGIENSIS基因与COOPOPTERAN活动公开(公告)号：US20150259702A1
公开(公告)日：2015-09-17
申请号：US14435699
申请日：2013-10-14
发明人： Takashi Yamamoto , Michi Izumi Willcoxon , Lu Liu , Ute Schellenberger , Eric Schepers , Yi Zheng
IPC分类号： C12N15/82 , C07K14/245 , A01N63/02 , C07K14/325
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/245 , C07K14/325 , C07K2319/55
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests including, but not limited to, insect pests belonging to the orders Coleoptera and Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, polynucleotide constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供了从编号多肽的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其对昆虫害虫具有杀虫活性，包括但不限于属于鞘翅目和鳞翅目鳞翅目的昆虫害虫。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，多核苷酸构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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公开(公告)号：US11959090B2
公开(公告)日：2024-04-16
申请号：US17242370
申请日：2021-04-28
发明人： Daniel J Altier , Jennifer Kara Barry , Ryan Michael Gerber , Steven D Gruver , Lu Liu , Ute Schellenberger , Jun-Zhi Wei , Weiping Xie , Nasser Yalpani , Genhai Zhu
IPC分类号： C12N15/82 , A01N37/46 , C07K14/195 , C07K14/21 , C07K14/265 , C07K14/32 , C07K14/33
CPC分类号： C12N15/8286 , A01N37/46 , C07K14/195 , C07K14/21 , C07K14/265 , C07K14/32 , C07K14/33
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US11008585B2
公开(公告)日：2021-05-18
申请号：US16098452
申请日：2017-05-02
发明人： Jennifer Kara Barry , Christian Bartholomay , Louisa D'Lima , James J. English , Kevin Robert Hayes , Lu Liu , Amy Lum , Brad Poland , Eric Jude Schepers , Weiping Xie , Nasser Yalpani , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US10563221B2
公开(公告)日：2020-02-18
申请号：US16001133
申请日：2018-06-06
发明人： Lu Liu , Jessica O'Rear , Young-Jun Park , Barbara Rosen , Ute Schellenberger , Eric Schepers , Jun-Zhi Wei , Weiping Xie , Nasser Yalpani , Xiaohong Zhong , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US09879277B2
公开(公告)日：2018-01-30
申请号：US14775344
申请日：2014-03-13
发明人： Andre R. Abad , Zhenglin Hou , Lu Liu , Brad Poland , Ute Schellenberger , Ingrid Udranszky , Jimei Wang , Jun-Zhi Wei , Thomas C. Wolfe , Weiping Xie , Lan Zhou , Genhai Zhu
CPC分类号： C12N15/8286 , A01N37/18 , A01N37/46 , A01N63/02 , C07K14/32 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are isolated insecticidal proteins and nucleic acids. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
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