公开(公告)号：US10519482B2

公开(公告)日：2019-12-31

申请号：US14381388

申请日：2013-02-28

Applicant: Becton, Dickinson and Company

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12Q1/24 ,  C12N1/02 ,  C12N1/20 ,  C12N1/04 ,  C12Q1/06

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

公开(公告)号：US10458887B2

公开(公告)日：2019-10-29

申请号：US15504549

申请日：2015-08-17

Applicant: Becton, Dickinson and Company

Inventor： Timothy Wiles ,  John D. Mantlo

IPC: G01N1/38 ,  G01N1/28 ,  G01N1/44 ,  G01N35/00 ,  G01N35/10 ,  G01N1/40 ,  H01J49/04 ,  H01J49/40 ,  G01N15/06

Abstract: Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).

公开(公告)号：US20150125895A1

公开(公告)日：2015-05-07

申请号：US14381388

申请日：2013-02-28

Applicant: Becton, Dickinson and Company

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12N1/20 ,  C12Q1/24

CPC classification number: C12Q1/14 ,  C12N1/02 ,  C12N1/20 ,  C12Q1/24

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Abstract translation: 本文公开的各种实施方案提供了用于从阳性血培养样品中快速分离存活的微生物细胞（包括肺炎链球菌）的试剂和方法。 所得到的微生物颗粒可用于鉴定和基于生长的方法如抗微生物敏感性测试。 本文所述的缓冲剂可以含有碱溶液，非离子洗涤剂，硫醇和任选的氯化铵。 所公开的方法提供了一种用于仅从一个样品制备管和离心同时从PBC样品中快速分离和浓缩活的微生物的方法，同时从哺乳动物血细胞中除去可能干扰鉴定方法的细胞碎片。

公开(公告)号：US20150118677A1

公开(公告)日：2015-04-30

申请号：US14390596

申请日：2013-04-04

Applicant: Becton, Dickinson and Company

Inventor： Xiao Li ,  Yongqiang Zhang ,  Kenneth Anthony Kopher ,  John D. Mantlo ,  William Alfred Pope ,  Song Shi ,  Axel A. Yup

IPC: C12Q1/06 ,  G01N1/30

CPC classification number: G01N1/30 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2001/302

Abstract: Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

Abstract translation: 本文描述了用于鉴定和分析样品中的至少一种微生物（例如细菌）的方法和试剂，并且通过流式细胞术分析样品时降低了获得的背景信号强度。 通过将样品与背景信号降低分子或与猝灭剂共价连接的核酸染色组合来制备样品。 在用核酸染色剂染色之前，可以用树脂去除样品中的一部分颗粒物质。