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    Methods for the Detection of Enterovirus D68 in Complex Samples
    12.
    发明申请
    Methods for the Detection of Enterovirus D68 in Complex Samples 审中-公开

    公开(公告)号：US20190024194A1

    公开(公告)日：2019-01-24

    申请号：US16041711

    申请日：2018-07-20

    Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

    Inventor： David Engelthaler ,  Jolene Bowers

    IPC: C12Q1/70

    Abstract: A method of detecting Enterovirus D68 is provided. The method may include adding to a mixture containing the sample from the subject, (a) a first forward primer comprising SEQ ID NO: 1, (b) a second forward primer comprising SEQ ID NO: 2, (c) a third forward primer comprising SEQ ID NO: 3, (d) a first reverse primer comprising SEQ ID NO: 4, and (e) a second reverse primer comprising SEQ ID NO: 5, subjecting the mixture to conditions that allow nucleic acid amplification, and detecting the presence or absence of Enterovirus D68 by analyzing the nucleic acid amplification products. The forward primers may include a first universal tail sequence and reverse primers may include a second universal tail sequence. The nucleic acid amplification products may be sequenced using next-generation sequencing.

    METHODS AND KITS FOR THE DETECTION OF SARS-COV-2
    14.
    发明申请
    METHODS AND KITS FOR THE DETECTION OF SARS-COV-2 有权

    公开(公告)号：US20230130878A1

    公开(公告)日：2023-04-27

    申请号：US17911341

    申请日：2021-03-12

    Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

    Inventor： David Engelthaler ,  Jolene Bowers ,  James Schupp

    IPC: C12Q1/70

    Abstract: Methods, kits, and oligonucleotides used in the detection of coronavirus, for example, SARS-CoV-2, CoV-HKU1, CoV-OC43, CoV-229E, and CoV-NL63, are disclosed. A method of detecting coronavirus may include contacting a sample with at least one primer pair and probe targeting SARS-CoV-2 and at least one of: a primer pair and probe targeting CoV-HKU1, a primer pair and robe targeting CoV-OC43, a primer pair and probe targeting CoV-229E, a primer pair and probe targeting CoV-NL63, subjecting the mixture to conditions that allow nucleic acid amplification, and detecting the presence or absence of coronavirus, including SARS-CoV-2, CoV-HKU1, CoV-OC43, CoV-229E, and/or CoV-NL63, by analyzing the nucleic acid amplification products.

    METHODS AND KITS FOR THE DETECTION OF SARS-COV-2
    15.
    发明申请
    METHODS AND KITS FOR THE DETECTION OF SARS-COV-2 有权

    公开(公告)号：US20230128191A1

    公开(公告)日：2023-04-27

    申请号：US17911350

    申请日：2021-03-12

    Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

    Inventor： David Engelthaler ,  Jolene Bowers ,  James Schupp

    IPC: C12Q1/70

    Abstract: Methods, kits, and oligonucleotides used in the detection of the coronavirus strain, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are disclosed. In some aspects, the oligonucleotides are primers or probes used in the described methods or kits. The oligonucleotide consists of 42 or less nucleotides and has a nucleotide sequence that consists essentially of, or is a variant of, the nucleotide sequence of: SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7. In some embodiments, the oligonucleotide is modified with an internal spacer or a detectable label. For example, the 5′ terminus is labeled with a fluorophore and the 3′ terminus is complexed to a quencher of fluorescence of said fluorophore. In some embodiments, the nucleotide sequence of the oligonucleotide further comprises a universal tail sequence.

    EARLY DETECTION OF DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS
    17.
    发明申请
    EARLY DETECTION OF DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS 有权

    公开(公告)号：US20220145366A1

    公开(公告)日：2022-05-12

    申请号：US17351172

    申请日：2021-06-17

    Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

    Inventor： David Engelthaler ,  Christopher Allender ,  James Schupp

    IPC: C12Q1/689 ,  C12Q1/686

    Abstract: The present invention relates to oligonucleotides, methods, and kits useful for detecting an antibiotic-resistant subpopulation within a heteroresistant population of Mycobacterium tuberculosis in a sample. An amplicon of a target locus is obtained from the sample. The target locus comprises a region of interest which comprises one or more minor variants associated with the antibiotic resistance. The target locus is selected from the group consisting of: pncA, tlyA, gidB, rpsL, gyrB, embB, ahpC promoter, rplC, and combinations thereof. The amplicon is sequenced on a Next Generation Sequencing (NGS) platform. The region of interest is interrogated to detect the one or more minor variants and thus, the antibiotic-resistant subpopulation of Mycobacterium tuberculosis.

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