公开(公告)号：US20180354987A1

公开(公告)日：2018-12-13

申请号：US15619148

申请日：2017-06-09

Applicant: General Electric Company

Inventor： Erik Leeming Kvam ,  Bruce Allan Hay ,  Ernest William Kovacs

IPC: C07K1/36 ,  C12N15/115 ,  C08F20/56 ,  C08L71/02 ,  C08L33/26 ,  C07K16/00 ,  B01D11/04

Abstract: A method for purification of a target in a solution includes providing a target in a solution, the solution comprising one or more contaminants, providing a polymer conjugate in the solution, wherein the polymer conjugate is configured to specifically bind the target, incubating the solution at a first temperature to facilitate binding of the polymer conjugate to the target, providing an environmentally-responsive block copolymer in the solution, wherein the environmentally-responsive block copolymer comprises one or more of an ethylene oxide (EO), a propylene oxide (PO), or an EO/PO block copolymer, and heating the solution comprising the environmentally-responsive block copolymer and the polymer conjugate bound to the target at a second temperature to initiate a liquid-liquid phase separation, wherein the liquid-liquid phase separation produces an aqueous phase and a liquid polymer phase, and wherein the liquid polymer phase comprises the polymer conjugate bound to the target.

公开(公告)号：US20140057282A1

公开(公告)日：2014-02-27

申请号：US14071805

申请日：2013-11-05

Applicant: General Electric Company

Inventor： Aaron Joseph Dulgar-Tulloch ,  Ernest William Kovacs ,  Evelina Roxana Loghin ,  Anup Sood

IPC: G01N1/34 ,  C07K14/00

CPC classification number: G01N1/34 ,  C07K14/001 ,  C12N2539/10 ,  G01N33/5375 ,  G01N33/54333

Abstract: Methods and kits for binding and releasing biological targets, comprising, a binder having an environmentally reactive molecular switch that can switch between a high affinity state, to bind the target, to a low affinity state, to release the target. In one embodiment, the binder is a pre-existing chemical sequence, composition, or structural configuration but unknown or unspecified and still viable as a binder for attaching a molecular switch.

Abstract translation: 用于结合和释放生物靶的方法和试剂盒，包括具有环境反应性分子开关的粘合剂，所述粘合剂可以在高亲和力状态之间切换以将靶结合到低亲和性状态以释放靶。 在一个实施方案中，粘合剂是预先存在的化学序列，组合物或结构构型，但是未知的或未指定的，并且作为用于连接分子开关的粘合剂仍然可行。