公开(公告)号：US20140178978A1

公开(公告)日：2014-06-26

申请号：US14049155

申请日：2013-10-08

Applicant: COLORADO STATE UNIVERSITY RESEARCH FOUNDATION

Inventor： David M. Cate ,  Josephine C. Cunningham ,  Charles S. Henry ,  John Volckens

IPC: G01N33/52 ,  G01N21/78 ,  C12Q1/54

CPC classification number: G01N33/521 ,  C12Q1/54 ,  G01N21/78 ,  G01N2021/0346

Abstract: Apparatus for quantitative analytical measurements using capillarity-based analytical devices is described. Porous cellulose (i.e., common filter paper) may be used as the reagent carrier for the analyses. Hydrophobic materials may be printed onto the paper to generate paths that restrict liquid flow by capillary action to defined regions. At least one colorimetric reagents effective for reacting with a specific analyte is deposited along a capillary flow path generated in the device. Upon placing the liquid containing the analyte on one end of the path, the liquid moves along the circuit by capillary action, and the flowing analyte reacts with reagent generating color along the flow path until all of the analyte is consumed. Analyte quantification is achieved by measuring the length of the colored portion along a flow path employing a direct-reading measurement scale. Analyses are demonstrated for chemical detection modalities including: enzymatic action, metal complexation, and nanoparticle aggregation, but the present apparatus may be used for a wide range of analytes.

Abstract translation: 描述了使用基于毛细管作用的分析装置进行定量分析测量的装置。 可以使用多孔纤维素（即普通滤纸）作为用于分析的试剂载体。 疏水材料可以印刷到纸上以产生通过毛细作用将液体流动限制到限定区域的路径。 至少一种有效与特定分析物反应的比色试剂沿着在器件中产生的毛细管流路沉积。 当将含有分析物的液体放置在路径的一端时，液体通过毛细作用沿着电路移动，并且流动的分析物与沿着流动路径产生颜色的试剂反应，直到所有的分析物被消耗。 通过使用直读测量标尺测量沿着流动路径的着色部分的长度来实现分析物定量。 对化学检测方式进行了分析，包括：酶促作用，金属络合和纳米颗粒聚集，但本装置可用于广泛的分析物。