公开(公告)号：US20100161733A1

公开(公告)日：2010-06-24

申请号：US12340088

申请日：2008-12-19

Applicant: Jason Michael Bower ,  Rui Li ,  Kenichi Morimoto ,  Honghui Sun ,  Simon Liu

Inventor： Jason Michael Bower ,  Rui Li ,  Kenichi Morimoto ,  Honghui Sun ,  Simon Liu

IPC: G06F15/82

CPC classification number: G06F17/276 ,  G06F3/0237 ,  G06F17/273 ,  G10L2015/228

Abstract: Word predictions in a message are selected or prioritized based on the recipient of the message and a previous location of use by a user. An input history is created based on messages sent to the recipient from the user at a particular location (e.g., global positioning system coordinates). As the user composes subsequent messages, a current location of the user is determined. Word predictions are performed based on a comparison of the current location to the previous locations, and based on the recipient(s). In further embodiments, location-aware spell-check functionality is provided for the messages.

Abstract translation: 基于消息的接收者和用户使用的先前位置来选择消息中的字预测或优先级。 基于在特定位置（例如，全球定位系统坐标）从用户发送到接收者的消息创建输入历史。 当用户组成后续消息时，确定用户的当前位置。 基于当前位置与先前位置的比较以及基于收件人的方式执行词预测。 在另外的实施例中，为消息提供了位置感知拼写检查功能。

公开(公告)号：US20090023226A1

公开(公告)日：2009-01-22

申请号：US12021074

申请日：2008-01-28

Applicant: M. Amin Arnaout ,  Rui Li ,  Jian-Ping Xiong

Inventor： M. Amin Arnaout ,  Rui Li ,  Jian-Ping Xiong

IPC: G01N33/566 ,  C07K16/18

CPC classification number: C07K14/70553 ,  A61K38/00 ,  C07K14/70546 ,  C07K16/2845

Abstract: Polypeptides comprising all or part of a variant integrin α subunit A domain and its flanking region are described. In solution or in membrane-associated form, the A domain polypeptides of the invention exists predominantly in a high affinity conformation. In the polypeptides of the invention, referred to as variant integrin polypeptides, a crucial isoleucine or glutamic acid residue is altered. For example, the glutamic acid can be either deleted or replaced with different amino acids residue, e.g., glutamine, aspartic acid, or alanine The variant integrin polypeptides of the invention selectively impair binding of activation-dependent ligands, but not independent ligands. They are useful in screening assays for the identification of molecules that enhance binding of variant polypeptides with impaired binding. In addition, they are useful in distinguishing between activation-dependent ligands and activation-independent ligands. They are also useful for generating antibodies, e.g., monoclonal antibodies, which bind to the impaired form of an integrin. Some such antibodies recognize an epitope that is either not present or not accessible on an integrin that is in the high affinity conformation. The variant integrin polypeptides of the invention can be derived from any integrin α subunit that could be used therapeutically.

Abstract translation: 描述了包含全部或部分变体整联蛋白α亚基A结构域及其侧翼区的多肽。 在溶液或膜相关形式中，本发明的A结构域多肽主要存在于高亲和力构象中。 在本发明的多肽中，称为变体整联蛋白多肽，关键的异亮氨酸或谷氨酸残基被改变。 例如，谷氨酸可被删除或被不同氨基酸残基替代，例如谷氨酰胺，天冬氨酸或丙氨酸。本发明的变体整联蛋白多肽选择性地损害活化依赖性配体但不是独立配体的结合。 它们可用于鉴定增强变异多肽与受损结合的结合的分子的筛选分析。 此外，它们可用于区分活化依赖性配体和与激活无关的配体。 它们也可用于产生结合受损形式的整联蛋白的抗体，例如单克隆抗体。 一些这样的抗体识别在高亲和力构象的整联蛋白上不存在或不可接近的表位。 本发明的变体整联蛋白多肽可以衍生自可以在治疗上使用的任何整联蛋白α亚基。

公开(公告)号：US07323552B2

公开(公告)日：2008-01-29

申请号：US10144259

申请日：2002-05-10

Applicant: M. Amin Arnaout ,  Rui Li ,  Jian-Ping Xiong

Inventor： M. Amin Arnaout ,  Rui Li ,  Jian-Ping Xiong

IPC: C07K1/107 ,  A61K38/04 ,  G01N33/53

CPC classification number: C07K14/70553 ,  A61K38/00 ,  C07K14/70546 ,  C07K16/2845

Abstract: Polypeptides comprising all or part of a variant integrin α subunit A domain and its flanking region are described. In solution or in membrane-associated form, the A domain polypeptides of the invention exists predominantly in a high affinity conformation. In the polypeptides of the invention, referred to as variant integrin polypeptides, a crucial isoleucine or glutamic acid residue is altered. For example, the glutamic acid can be either deleted or replaced with different amino acids residue, e.g., glutamine, aspartic acid, or alanine The variant integrin polypeptides of the invention selectively impair binding of activation-dependent ligands, but not independent ligands. They are useful in screening assays for the identification of molecules that enhance binding of variant polypeptides with impaired binding. In addition, they are useful in distinguishing between activation-dependent ligands and activation-independent ligands. They are also useful for generating antibodies, e.g., monoclonal antibodies, which bind to the impaired form of an integrin. Some such antibodies recognize an epitope that is either not present or not accessible on an integrin that is in the high affinity conformation. The variant integrin polypeptides of the invention can be derived from any integrin α subunit that could be used therapeutically.

Abstract translation: 描述了包含全部或部分变体整联蛋白α亚基A结构域及其侧翼区的多肽。 在溶液或膜相关形式中，本发明的A结构域多肽主要存在于高亲和力构象中。 在本发明的多肽中，称为变体整联蛋白多肽，关键的异亮氨酸或谷氨酸残基被改变。 例如，谷氨酸可被删除或被不同氨基酸残基替代，例如谷氨酰胺，天冬氨酸或丙氨酸。本发明的变体整联蛋白多肽选择性地损害活化依赖性配体但不是独立配体的结合。 它们可用于鉴定增强变异多肽与受损结合的结合的分子的筛选分析。 此外，它们可用于区分活化依赖性配体和与激活无关的配体。 它们也可用于产生结合受损形式的整联蛋白的抗体，例如单克隆抗体。 一些这样的抗体识别在高亲和力构象的整联蛋白上不存在或不可接近的表位。 本发明的变体整联蛋白多肽可以衍生自可以在治疗上使用的任何整联蛋白α亚基。