公开(公告)号：US11767537B2

公开(公告)日：2023-09-26

申请号：US15754881

申请日：2016-08-25

申请人： LONZA BIOLOGICS PLC.

发明人： Robert Young ,  James D. Budge ,  Mark C. Smales

IPC分类号： C12N15/85 ,  C07K14/005 ,  C07K16/00 ,  C12N5/071 ,  C12N7/00 ,  C12N9/12 ,  C12N15/67

CPC分类号： C12N15/85 ,  C07K14/005 ,  C07K16/00 ,  C12N5/0682 ,  C12N7/00 ,  C12N9/1229 ,  C12N15/67 ,  C12Y207/04004 ,  C12N2800/108

摘要： The present invention relates to an expression system for the heterologous expression of a nucleic acid sequence of interest in a mammalian cell, the system comprising: (i) a first genetic entity, comprising: a nucleic acid sequence encoding a functional Epstein Barr virus nuclear antigen 1 (EBNA-1), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional EBNA-1; (ii) a second genetic entity, comprising: a nucleic acid sequence encoding a functional nucleoside diphosphate kinase A (NDPK-A), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional NDPK-A; (iii) a third genetic entity, comprising: the nucleic acid sequence of interest being operably linked to regulatory elements that allow for expression of the nucleic acid sequence of interest; and (iv) a four genetic entity, comprising: the Epstein Barr virus OriP sequence or one or more subsequences thereof, wherein the one or more subsequences comprise at least the ‘Family of Repeats’ DNA-binding site for EBNA-1 and the ‘Dyad Symmetry’ DNA-binding site for EBNA-1. The present invention also relates to corresponding mammalian host cells and methods for expressing a nucleic acid sequence of interest by means of such expression system.