公开(公告)号：US11661629B2

公开(公告)日：2023-05-30

申请号：US16666188

申请日：2019-10-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Hendricks ,  David J. King

IPC: C12Q1/6874 ,  A61J1/06 ,  A61J1/14 ,  B65B3/00 ,  B65B31/04 ,  B65D8/00 ,  C12N9/00 ,  C12Q1/6876 ,  A61M5/00 ,  A61M5/50 ,  B65B55/08 ,  B65B55/10

CPC classification number: C12Q1/6874 ,  A61J1/062 ,  A61J1/14 ,  B65B3/003 ,  B65B3/006 ,  B65B31/046 ,  B65D15/02 ,  C12N9/93 ,  C12Q1/6876 ,  A61J2200/76 ,  A61J2205/30 ,  A61M5/008 ,  A61M5/5086 ,  B65B55/08 ,  B65B55/10 ,  C12Q2600/16 ,  C12Y605/01001

Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.