公开(公告)号：US20180223359A1

公开(公告)日：2018-08-09

申请号：US15945348

申请日：2018-04-04

Applicant: Life Technologies Corporation

Inventor： Steven M. MENCHEN ,  Alan BLANCHARD ,  Luisa ANDRUZZI ,  Shaheer H. KHAN ,  Dmitriy GREMYACHINSKIY ,  Alfred LUI ,  Craig STOLARCZYK ,  Tanya SOKOLSKY ,  Prasanna Krishnan THWAR

IPC: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US20230407391A1

公开(公告)日：2023-12-21

申请号：US18209766

申请日：2023-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. MENCHEN ,  Alan BLANCHARD ,  Luisa ANDRUZZI ,  Shaheer KHAN ,  Dmitriy GREMYACHINSKIY ,  Alfred LUI ,  Craig STOLARCZYK ,  Tanya SOKOLSKY ,  Prasanna Krishnan THWAR

IPC: C12Q1/6876 ,  C12Q1/6834 ,  C08F8/40

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C08F8/40

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US20160188795A1

公开(公告)日：2016-06-30

申请号：US14952001

申请日：2015-11-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marcin Sikora ,  Alan BLANCHARD

IPC: G06F19/22 ,  C12Q1/68

CPC classification number: G16B25/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  G16B30/00 ,  G16B99/00 ,  C12Q2565/102 ,  C12Q2521/501

Abstract: Disclosed are systems and methods for polynucleotide sequencing where detection and correction of base calling errors can be achieved without reliance on a reference sequence. In certain embodiments, redundant information can be introduced during measurement so as to allow such detection of errors. Such redundant information and measurements can be facilitated by encoding of nucleotide sequence being measured. Various examples of such encoding, redundancy introduction, and decoding are provided.