公开(公告)号：US20210269871A1

公开(公告)日：2021-09-02

申请号：US17193232

申请日：2021-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Swati GOYAL ,  Achim KARGER ,  Peter MA ,  S. Jeffrey ROSNER ,  Ian WALTON ,  Jonathan WANG ,  Michael WENZ

IPC: C12Q1/6869 ,  B01L3/00 ,  C12Q1/6806

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

公开(公告)号：US20230314369A1

公开(公告)日：2023-10-05

申请号：US18108471

申请日：2023-02-10

Applicant: LIFE TECHNOLOGIES CORPORATION ,  INTEGENX INC.

Inventor： Jen-Kuei LIU ,  Bharti SOLANKI ,  Hans Michael WENZ ,  Frank MERCER ,  Cevat AKIN ,  Achim KARGER ,  Annelise BARRON ,  Scott MACK

IPC: G01N27/447 ,  C08L33/26

CPC classification number: G01N27/44747 ,  C08L33/26 ,  C08L2203/02 ,  C08L2205/025

Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.

公开(公告)号：US20200333291A1

公开(公告)日：2020-10-22

申请号：US16922754

申请日：2020-07-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ian WALTON ,  Robert COBENE ,  Alexander DUKHOVNY ,  Adam SANNICANDRO ,  John DIXON ,  Achim KARGER ,  Dan KLINE

IPC: G01N27/447 ,  G01N35/10

Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.