公开(公告)号：US20220113314A1

公开(公告)日：2022-04-14

申请号：US17495133

申请日：2021-10-06

Applicant: Korea Advanced Institute Of Science And Technology

Inventor： Jae-Byum CHANG ,  Yehlin CHO ,  Haemin YEON ,  Junyoung SEO ,  Yeonbo SIM

IPC: G01N33/58 ,  G01N33/53 ,  G01N21/64

Abstract: Various embodiments can provide a method of amplifying a fluorescence signal through the cyclic staining of complementary antibodies conjugated with fluorophores. According to various embodiments, the method of amplifying a fluorescence signal may be configured to prepare a primary antibody and antibody pairs with respect to each target protein, bind the primary antibody to the target protein, and bind the antibody pairs to the primary antibody. The multicolor fluorescence signals may be amplified and fabricated by forming fluorescence signals in a way to prepare different antibodies for which cross reactions with different types of target proteins, respectively, have been removed using an agarose gel and to bind different antibody pairs according to combinations of different antibodies to different primary antibodies bound to target proteins, respectively.

公开(公告)号：US20220065788A1

公开(公告)日：2022-03-03

申请号：US17445629

申请日：2021-08-23

Applicant: Korea Advanced Institute Of Science And Technology

Inventor： Jae-Byum CHANG ,  Young-Gyu YOON ,  Junyoung SEO ,  Yeonbo SIM ,  Jee Won KIM

IPC: G01N21/64

Abstract: The present disclosure provides a method and apparatus for biomolecular multiplexed imaging through the iterative unmixing of fluorophores. According to the first embodiments of the present disclosure, although signals of two fluorophores are detected in the first fluorescent detection spectral range, the signals of the two images obtained from two detection spectral ranges can be unmixed through the iterative minimization of mutual information. Furthermore, the present disclosure provides a multi-color unmixing method and apparatus through the iterative minimization of mutual information. In the second embodiments of the present disclosure, a plurality of images of a plurality of fluorophores marking different biomolecules, respectively, for example, N fluorophores are obtained. Images, each containing the signals of single fluorophore can be obatined from the obtained images while minimizing the mutual information shared by images of each of pairs each consisting of two of the obtained images.