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公开(公告)号：US10905107B2
公开(公告)日：2021-02-02
申请号：US15695417
申请日：2017-09-05
Inventor： Hee-Sung Park , Aerin Yang
IPC: A01K67/027 , C12N15/85 , C12N9/00
Abstract: The present invention relates to a mouse (Mus musculus) in which expression and site-specific modification of a target protein is temporally and spatially controlled, and a method for producing the same and the use thereof, and more particularly to a transgenic mouse in which expression of a target protein having a modification attached to a specific position is temporally and spatially controlled as a result of incorporation of an unnatural amino acid. In the mouse according to the present invention, in which site-specific modification of a target protein is temporally and spatially controllable, expression of the target protein having the site-specific modification attached thereto is controllable depending on the timing and/or position of introduction of an unnatural amino acid. Thus, the mouse according to the present invention is useful for studies on the in vivo functions of cellular proteins, various human diseases including cancers and neurodegenerative disorders, new drug discovery, and the like.
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2.发明授权公开(公告)号：US10723760B2
公开(公告)日：2020-07-28
申请号：US15692403
申请日：2017-08-31
Inventor： Hee-Sung Park , Hee-Yoon Lee , Aerin Yang , Sura Ha
Abstract: A method for producing a site-specifically modified protein based on new carbon-carbon bond formation is disclosed, including the following three steps (marking, activation, and coupling steps): (a) marking of the modification site by incorporating a specific amino acid into a selected position of a target protein; (b) activation of the marked site; and (c) coupling of various post-translational modification (PTM) moieties or other chemical groups onto the activated site to obtain a site-specifically modified protein. The method for producing a site-specifically modified protein can incorporate desired diverse chemical groups including post-translational modification (PTM) moieties into a designated site in a target protein through a new carbon-carbon bond. Furthermore, the modified protein having a site-specific PTM exhibits the same chemical and functional properties as that of a target protein present in cells. Thus, the present invention is useful for studies of cellular proteins, human diseases including cancers and neurodegenerative diseases, and new drug discovery.
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3.发明申请公开(公告)号：US20180066011A1
公开(公告)日：2018-03-08
申请号：US15692403
申请日：2017-08-31
Inventor： Hee-Sung Park , Hee-Yoon Lee , Aerin Yang , Sura Ha
Abstract: A method for producing a site-specifically modified protein based on new carbon-carbon bond formation is disclosed, including the following three steps (marking, activation, and coupling steps): (a) marking of the modification site by incorporating a specific amino acid into a selected position of a target protein; (b) activation of the marked site; and (c) coupling of various post-translational modification (PTM) moieties or other chemical groups onto the activated site to obtain a site-specifically modified protein. The method for producing a site-specifically modified protein can incorporate desired diverse chemical groups including post-translational modification (PTM) moieties into a designated site in a target protein through a new carbon-carbon bond. Furthermore, the modified protein having a site-specific PTM exhibits the same chemical and functional properties as that of a target protein present in cells. Thus, the present invention is useful for studies of cellular proteins, human diseases including cancers and neurodegenerative diseases, and new drug discovery.
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公开(公告)号：US20180064076A1
公开(公告)日：2018-03-08
申请号：US15695417
申请日：2017-09-05
Inventor： Hee-Sung Park , Aerin Yang
IPC: A01K67/027 , C12N9/00 , C12N15/85
CPC classification number: A01K67/0275 , A01K2217/072 , A01K2217/15 , A01K2217/20 , A01K2217/206 , A01K2227/105 , A01K2267/03 , C12N9/93 , C12N15/8509 , C12Y601/01006
Abstract: The present invention relates to a mouse (Mus musculus) in which expression and site-specific modification of a target protein is temporally and spatially controlled, and a method for producing the same and the use thereof, and more particularly to a transgenic mouse in which expression of a target protein having a modification attached to a specific position is temporally and spatially controlled as a result of incorporation of an unnatural amino acid. In the mouse according to the present invention, in which site-specific modification of a target protein is temporally and spatially controllable, expression of the target protein having the site-specific modification attached thereto is controllable depending on the timing and/or position of introduction of an unnatural amino acid. Thus, the mouse according to the present invention is useful for studies on the in vivo functions of cellular proteins, various human diseases including cancers and neurodegenerative disorders, new drug discovery, and the like.
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