公开(公告)号：US20230204515A1

公开(公告)日：2023-06-29

申请号：US18087341

申请日：2022-12-22

Applicant: HORIBA INSTRUMENTS INCORPORATED

Inventor： Nicolas VEZARD ,  Beth FINAMORE ,  Aashish TULADHAR

IPC: G01N21/65 ,  G01N21/64

CPC classification number: G01N21/65 ,  G01N21/6428 ,  G01N2201/06113 ,  G01N2201/0633 ,  G01N2201/0636 ,  G01N2201/08 ,  G01N2201/0635 ,  G01N2201/127

Abstract: A microplate reader simultaneously obtains Raman measurements from samples contained in non-adjacent wells. At least two Raman probes are positioned perpendicularly above or below the microplate to simultaneously acquire Raman spectra data of the non-adjacent liquid samples. Each probe is coupled to a laser and a spectrometer and includes a lens focusing laser light within the sample and collecting light from the sample for the spectrometer. The spectrometer may include a 2D imaging sensor (sCMOS or CCD) to image light from multiple probes simultaneously, spaced from one another to reduce crosstalk. A positioner moves the microplate plate or probes to acquire data from a different subset of non-adjacent samples, and may also vary laser focus within wells during data acquisition. Multiple fluorescence probes may simultaneously acquire fluorescence data from the same samples, or non-adjacent samples. Probes may be fiber-coupled and positioned within a reaction chamber of a liquid handling system.

公开(公告)号：US20170010153A1

公开(公告)日：2017-01-12

申请号：US15115497

申请日：2015-01-30

Applicant: HORIBA INSTRUMENTS INCORPORATED

Inventor： Nicolas VEZARD ,  Beth FINAMORE ,  Jeremy GOLDSTONE ,  Gregoire VERRIER

IPC: G01J3/02 ,  G01J3/04 ,  G01J3/06 ,  G01J3/28 ,  G01J3/18

CPC classification number: G01J3/0229 ,  G01J3/0202 ,  G01J3/0218 ,  G01J3/0291 ,  G01J3/04 ,  G01J3/06 ,  G01J3/18 ,  G01J3/2823 ,  G01J3/36 ,  G01J2003/064 ,  G01J2003/1828

Abstract: A system and method for spectroscopic mapping, with configurable spatial resolution, of an object include a fiber optic bundle having a plurality of optical fibers arranged in a first array at an input end with each of the plurality of optical fibers spaced one from another and arranged in at least one linear array at an output end. A first mask defining a plurality of apertures equal to or greater in number than the plurality of optical fibers is positioned between an object to be imaged and the input end of the fiber optic bundle. An imaging spectrometer is positioned to receive light from the output end of the fiber optic bundle and to generate spectra of the object. A sensor associated with the imaging spectrometer converts the spectra to electrical output signals for processing by an associated computer.

Abstract translation: 具有可配置空间分辨率的对象的光谱映射的系统和方法包括具有多个光纤的光纤束，所述光纤束具有在输入端以第一阵列布置的多个光纤，所述多个光纤中的每一个彼此间隔开并布置 在输出端的至少一个线性阵列。 限定数量等于或大于多个光纤的多个孔的第一掩模位于待成像对象与光纤束的输入端之间。 成像光谱仪定位成从光纤束的输出端接收光并产生物体的光谱。 与成像光谱仪相关联的传感器将光谱转换成电输出信号以供相关计算机处理。