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公开(公告)号:US09695431B2
公开(公告)日:2017-07-04
申请号:US15024863
申请日:2014-09-25
发明人: Bashir Mohammad Khan , Neha Gupta , Parth Sanjaykumar Patel , Poonam Sharma , Shuchishweta Vinay Kendurkar
IPC分类号: C12N15/82 , C07K14/41 , C12N15/84 , C07K14/415
CPC分类号: C12N15/8205 , C07K14/415 , C12N15/8243
摘要: Described herein is a process of genetic transformation in W. somnifera by Agrobacterium tumefaciens mediated transformation to overexpress squalene synthase gene (WsSQS) encoding WsSQS enzyme that catalyzes the synthesis of squalene from farnesyl pyrophosphate. Increased withanolide level including withaferin-A, withanolide A and B and withanone is attained in transformed plant tissues.