公开(公告)号：US20180136107A1

公开(公告)日：2018-05-17

申请号：US15870661

申请日：2018-01-12

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Sean Lee ,  Kilo Vilayphone ,  Xiaobo Wang

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A method of collecting cells from individual wells of a multi-well plate for flow cytometry, the method comprising: determining particle or cell counts from each well of the multi-well plate by flow cytometry; ordering the wells from lowest particle count to highest particle count; generating a sequential order for collection that follows the ordering of wells to establish a collection pattern; and collecting particles or cells according to the collection pattern in a second multi-well plate by flow cytometry.

公开(公告)号：US09869628B2

公开(公告)日：2018-01-16

申请号：US14750613

申请日：2015-06-25

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Sean Lee ,  Kilo Vilayphone ,  Xiaobo Wang

IPC: G01N15/14 ,  G01N15/10

CPC classification number: G01N15/1404 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A method of collecting cells from individual wells of a multi-well plate for use in flow cytometry, the method including adding a suspension of cells to wells of the multi-well plate; and aspirating cells from different wells according to a collection pattern into a flow cytometer, wherein the collection pattern is a sequential ordering of wells beginning at a middle region of the multi-well plate and continuing towards an outer region of the multi-well plate. The method preferably including rotating or agitating the multi-well plate between steps of aspirating cells from different wells. Exemplary collection patterns include spiral-square collection pattern and a nearest well to center collection pattern.

公开(公告)号：US20160097707A1

公开(公告)日：2016-04-07

申请号：US14750613

申请日：2015-06-25

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Sean Lee ,  Kilo Vilayphone ,  Xiaobo Wang

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A method of collecting cells from individual wells of a multi-well plate for use in flow cytometry, the method including adding a suspension of cells to wells of the multi-well plate; and aspirating cells from different wells according to a collection pattern into a flow cytometer, wherein the collection pattern is a sequential ordering of wells beginning at a middle region of the multi-well plate and continuing towards an outer region of the multi-well plate. The method preferably including rotating or agitating the multi-well plate between steps of aspirating cells from different wells. Exemplary collection patterns include spiral-square collection pattern and a nearest well to center collection pattern.

Abstract translation: 一种从多孔板的单个孔中收集细胞用于流式细胞术的方法，所述方法包括向多孔板的孔中加入细胞悬浮液; 以及根据收集模式将不同孔中的细胞抽吸到流式细胞仪中，其中所述收集模式是从所述多孔板的中间区域开始并且朝向所述多孔板的外部区域继续的孔的连续排序。 该方法优选地包括在从不同孔抽吸细胞的步骤之间旋转或搅拌多孔板。 示例性收集模式包括螺旋形收集模式和最近的井到中心收集模式。