Abstract:
Disclosed herein are an Arabidopsis gene (AtLEJ1), acting as an inhibitor of the biosynthesis of the phytohormones jasmonic acid and ethylene, and a method for producing a male sterile transgenic plant using the same. The gene guarantees stable male sterile strains which need no maintainers for the maintenance thereof. Also, the transgenic plants comprising the gene can be readily restored to a fertile phenotype merely through hormonal treatment. Although the preferred embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.
Abstract:
Methods for enhancing various economically important yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a LEJ1 (Loss of timing of ET and JA biosynthesisI) polypeptide, ExbB polypeptide, NMPRT (nicotinamide phosphoribosyltransferase) polypeptide, AP2-26-like polypeptide or HD8-like polypeptide are provided. Plants produced by the methods are also provided, which have enhanced yield-related traits relative to corresponding wild type plants or other control plants. Constructs comprising a nucleic acid encoding a LEJ1, ExbB, NMPRT, AP2-26-like or HD8-like polypeptide and uses thereof are provided.
Abstract:
Disclosed herein are a sweetpotato expansin cDNA (IbExpansin), a plant transformation vector carrying the same, and a transgenic plant comprising the vector. The transgenic Arabidopsis, prepared using the IbExpansin cDNA, outgrows the wild-type, thereby increasing in size and seed production up to three times. Thus, the gene is useful in the generation of highly productive transgenic plants for improvement in biomass and/or seed production.
Abstract:
A laticiferous tissue-specific SRPP (small rubber particle-associated protein) promoter derived from Hevea brasiliensis, consists of nucleotide sequence of SEQ ID NO: 1. A recombinant plant expression vector includes the promoter. A plant is transformed with the recombinant plant expression vector and seed of the transformed plant is obtained. A method for laticiferous tissue-specific expression of a foreign gene in a transformed plant includes performing recombination of a foreign gene into the recombinant plant expression vector. The transformed plant produced by the method shows laticiferous tissue-specific expression of a foreign gene.
Abstract translation:来源于巴西三叶草的起绒组织特异性SRPP(小橡胶颗粒相关蛋白)启动子由SEQ ID NO:1的核苷酸序列组成。重组植物表达载体包含启动子。 用重组植物表达载体转化植物,获得转化植物的种子。 用于转基因植物中外源基因的皮肤组织特异性表达的方法包括将外源基因重组进入重组植物表达载体中。 通过该方法产生的转化植物显示外源基因的有丝分裂的组织特异性表达。
Abstract:
Disclosed herein are antisense DNA of a sweetpotato expansin (IbExpansin) cDNA, a plant transformation vector carrying the same, and a method for increasing storage root production using the same. The transgenic sweetpotatoes prepared using the antisense DNA of IbExpansin cDNA have storage root production increased by up to one and half times. Thus, the gene is useful in the generation of highly productive transgenic storage roots for the increase of bioethanol production.
Abstract:
Disclosed herein are antisense DNA of a sweetpotato expansin (IbExpansin) cDNA, a plant transformation vector carrying the same, and a method for increasing storage root production using the same. The transgenic sweetpotatoes prepared using the antisense DNA of IbExpansin cDNA have storage root production increased by up to one and half times. Thus, the gene is useful in the generation of highly productive transgenic storage roots for the increase of bioethanol production.
Abstract:
A laticiferous tissue-specific SRPP (small rubber particle-associated protein) promoter derived from Hevea brasiliensis, consists of nucleotide sequence of SEQ ID NO: 1. A recombinant plant expression vector includes the promoter. A plant is transformed with the recombinant plant expression vector and seed of the transformed plant is obtained. A method for laticiferous tissue-specific expression of a foreign gene in a transformed plant includes performing recombination of a foreign gene into the recombinant plant expression vector. The transformed plant produced by the method shows laticiferous tissue-specific expression of a foreign gene.
Abstract translation:来源于巴西三叶草的起绒组织特异性SRPP(小橡胶颗粒相关蛋白)启动子由SEQ ID NO:1的核苷酸序列组成。重组植物表达载体包含启动子。 用重组植物表达载体转化植物,获得转化植物的种子。 用于转基因植物中外源基因的皮肤组织特异性表达的方法包括将外源基因重组进入重组植物表达载体中。 通过该方法产生的转化植物显示外源基因的有丝分裂的组织特异性表达。
Abstract:
Disclosed herein are a novel sucrose-inducible promoter sequence and a 5′ untranslated region which are derived from sweetpotato ADP-glucose pyrophosphorlyase gene (ibAGP1) (SEQ ID NO: 1). Also disclosed are expression vectors using the same sequences and a transgenic plant using the same vectors. The promoter and 5′ untranslated region according to the present invention can confer a high level of sucrose-inducible expression in plants, particularly in plant storage roots which contain sucrose in relatively large quantities to accumulate starch in large quantities in plants. Therefore the present invention may be useful for the generation of transgenic plants to produce useful proteins in large quantities in plant storage roots.
Abstract translation:本文公开了衍生自甘薯ADP-葡萄糖焦磷酸酶基因(ibAGP1)(SEQ ID NO:1)的新型蔗糖诱导型启动子序列和5'非翻译区。 还公开了使用相同序列的表达载体和使用相同载体的转基因植物。 根据本发明的启动子和5'非翻译区可以在植物中特别是含有相对大量的蔗糖的植物贮藏根中能够在植物中大量积聚淀粉而赋予高水平的蔗糖诱导表达。 因此,本发明可用于产生转基因植物以在植物贮藏根中大量生产有用的蛋白质。
Abstract:
Disclosed herein are an Arabidopsis gene (AtLEJ1), acting as an inhibitor of the biosynthesis of the phytohormones jasmonic acid and ethylene, and a method for producing a male sterile transgenic plant using the same. The gene guarantees stable male sterile strains which need no maintainers for the maintenance thereof. Also, the transgenic plants comprising the gene can be readily restored to a fertile phenotype merely through hormonal treatment. Although the preferred embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.
Abstract:
Disclosed herein is a novel gene which is isolated from Arabidopsis thaliana and shows senescence-specific expression. The expression of the gene is under the regulation of a promoter which is also disclosed herein. The gene in combination with the promoter can be utilized at the molecular level to control plant senescence in an environmentally friendly manner.