The present invention relates to the field of display technology, particularly to a pixel defining layer, an organic electroluminescent device and a display device. The pixel defining layer comprises a first defining layer on an array substrate and a second defining layer on the first defining layer, wherein the first defining layer is made of a lyophilic inorganic material, and the second defining layer is made of a lyophobic organic material. The present invention provides a pixel defining layer, an organic electroluminescent device and a display device, wherein ink-jet printing technology is used to patternize pixels of an organic material, which serves as not only a protective layer on the first inorganic material layer during dry etching, but also a lyophobic layer on the second layer; the making of the double-layered pixel defining layer omits the process of using two masks, which can effectively reduce costs and improve production efficiency.