The present invention relates to a new Physical layer Protocol Data unit (PPDU) format and a transmission and reception method and apparatus using the new PPDU format in a Wireless Local Area Network (WLAN). According to one aspect of the present invention, a method for transmitting a PPDU frame to a reception apparatus by a transmission apparatus in a WLAN may be provided. The method may include generating a legacy preamble, generating a High Efficiency (HE)-preamble, and transmitting a PPDU frame including a first region and a second region to the reception apparatus, the first region including the legacy preamble and a first field and the second region including the HE-preamble. The first field may have a variable length, and the HE-preamble may include a High Efficiency-Short Training Field (HE-STF) following the first field.