A display screen and a method of preparing the same are disclosed. The method includes steps of: forming a flexible base layer (20) on a rigid base substrate (10), wherein the rigid base substrate (10) includes a first region (101), a second region (102), and a connection region (103) between the first region and the second region, and the flexible base layer (20) is at least formed in both the second region (102) and the connection region (103); fabricating an organic light emitting diode device on the substrate with the flexible base layer (20) formed thereon; and stripping the rigid base substrate (10) in the second region (102) along a boundary between the second region (102) and the connection region (103). By means of a flexible display technology, the above method can achieve seamless assembly between adjacent sub-screens, and therefore increases display quality of pictures.