A tape spindle comprising a first holding portion and a second holding portion individually supporting and fastening to tape rolls of different sizes, wherein the second holding portion is constructed by a column with at least two orthogonal diagonals, and an operating section of the tape roll is extended through an opening of the first holding portion to reach a tape dispenser; therefore, the tape spindle may accommodate the purpose of the user to hold tape rolls on the first holding portion and the second holding portion according to their sizes, and when extending the tape roll held on the second holding portion, the shaft of the tape spindle may not move with the rotation of the tape roll due to the friction force between an inner wall of the tape roll and the second holding portion.