发明授权
- 专利标题: Enhanced expression method
- 专利标题(中): 增强表达方法
-
申请号: US12376312申请日: 2007-08-03
-
公开(公告)号: US09388421B2公开(公告)日: 2016-07-12
- 发明人: Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
- 申请人: Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
- 申请人地址: NO Trondheim
- 专利权人: VECTRON BIOSOLUTIONS AS
- 当前专利权人: VECTRON BIOSOLUTIONS AS
- 当前专利权人地址: NO Trondheim
- 代理机构: Porter, Wright, Morris & Arthur LLP
- 优先权: GB0615556.8 20070803
- 国际申请: PCT/GB2007/002951 WO 20070803
- 国际公布: WO2008/015447 WO 20080207
- 主分类号: C12N15/70
- IPC分类号: C12N15/70 ; C07H21/04
摘要:
The present invention concerns a method of producing a desired heterologous gene product wherein said heterologous gene product is expressed from a strong promoter, said method comprising expressing said gene using a mutant mRNA leader which comprises one or more mutations which enhance transcription of the gene. The invention also provides a mutant Pm mRNA leader sequence, and a vector and a library comprising the leader sequence. Methods of obtaining an mRNA mutant leader and identifying an mRNA mutant leader are encompassed, along with a vector for selection or identification of an mRNA leader mutant and a use thereof for screening.
公开/授权文献
- US20100248972A1 Enhanced Expression Method 公开/授权日:2010-09-30
信息查询
IPC分类: