发明授权
US09382581B2 Primers with modified phosphate and base in allele-specific PCR
有权
在等位基因特异性PCR中具有修饰的磷酸酯和碱基的引物
- 专利标题： Primers with modified phosphate and base in allele-specific PCR
- 专利标题（中）： 在等位基因特异性PCR中具有修饰的磷酸酯和碱基的引物
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申请号： US14092133申请日： 2013-11-27
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公开(公告)号： US09382581B2公开(公告)日： 2016-07-05
- 发明人: Stefanie Froehner , Dieter Heindl , Dirk Kessler , Nancy Schoenbrunner , Alison Tsan
- 申请人： Roche Molecular Systems, Inc.
- 申请人地址： US CA Pleasanton
- 专利权人： ROCHE MOLECULAR SYSTEMS, INC.
- 当前专利权人： ROCHE MOLECULAR SYSTEMS, INC.
- 当前专利权人地址： US CA Pleasanton
- 代理商 David J Chang
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating both a nucleotide with a base covalently modified at the exocyclic amino group and a modified phosphate.
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