What is disclosed is a system and method for determining a subject of interest's arterial pulse transit time from time-varying source signals generated from video images. In one embodiment, a video imaging system is used to capture a time-varying source signal of a proximal and distal region of a subject of interest. The image frames are processed to isolate localized areas of a proximal and distal region of exposed skin of the subject. A time-series signal for each of the proximal and distal regions is extracted from the source video images. A phase angle is computed with respect to frequency for each of the time-series signals to produce respective phase v/s frequency curves for each region. Slopes within a selected cardiac frequency range are extracted from each of the phase curves and a difference is computed between the two slopes to obtain an arterial pulse transit time for the subject.