发明授权
US09085768B2 Application of the broccoli wound-inducible promoter of glucose inhibition of root elongation 1 gene in transgenic plants
有权
在转基因植物中应用花椰菜伤口诱导型启动子葡萄糖抑制根伸长1基因
- 专利标题： Application of the broccoli wound-inducible promoter of glucose inhibition of root elongation 1 gene in transgenic plants
- 专利标题（中）： 在转基因植物中应用花椰菜伤口诱导型启动子葡萄糖抑制根伸长1基因
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申请号： US13572541申请日： 2012-08-10
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公开(公告)号： US09085768B2公开(公告)日： 2015-07-21
- 发明人: Wan-Hsing Cheng , Ming-Hau Chiang , Ya-Huei Chen , Hwei-Ling Shen
- 申请人： Wan-Hsing Cheng , Ming-Hau Chiang , Ya-Huei Chen , Hwei-Ling Shen
- 申请人地址： TW Taipei
- 专利权人： Academia Sinica
- 当前专利权人： Academia Sinica
- 当前专利权人地址： TW Taipei
- 代理机构： Drinker Biddle & Reath LLP
- 主分类号： C12N15/82
- IPC分类号： C12N15/82 ; C12N15/113 ; C07H21/04
摘要：
In this study, we used a wound-inducible promoter of the broccoli (Brassica oleracea var. italica) GLUCOSE INHIBITION of ROOT ELONGATION1 (GIR1) gene fused to β-glucuronidase (GUS, pBoGIR1::GUS) as a selectable marker. Transgenic broccoli plants expressing pBoGIR1::GUS appear blue in planta at wounded regions after GUS staining for 30 min. Similarly, the blue color is visible in transgenic Arabidopsis and rice plants expressing pBoGIR1::GUS at wounded areas after GUS staining for 2 h, indicating that this promoter is wound-inducible in both dicots and monocots. GUS staining is very rapid and the partial wounding in this study is a nondestructive method that does not affect further plant growth and development. Thus, pBoGIR1::GUS could serve as an effective substitute for antibiotic- and herbicide-resistance genes in the generation of genetically modified crops.
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