The present invention relates to a method and apparatus for supporting location registration and mobility for each service flow in relation to a Mobile Node (MN) having a plurality of wired/wireless network interfaces in a wired/wireless integrated network environment. A method includes receiving a location registration message from a specific Handover Control Agent (HCA) connected to an MN, determining a movement of a flow, configured in another HCA to which the MN is connected, based on predetermined static flow binding information or a network status, sending a location registration Ack. message, comprising information on a HCA in which the flow is configured and to which a Corresponding Node (CN) is connected, to the specific HCA, and sending a location notification message, comprising information on the specific HCA to which the flow will be moved and to which the CN is connected, to the HCA.