Invention Grant
US08895244B2 Method and kit for detecting 5-hydroxymethylcytosine in nucleic acids
有权
用于检测核酸中5-羟甲基胞嘧啶的方法和试剂盒
- Patent Title: Method and kit for detecting 5-hydroxymethylcytosine in nucleic acids
- Patent Title (中): 用于检测核酸中5-羟甲基胞嘧啶的方法和试剂盒
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Application No.: US14111825Application Date: 2012-04-16
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Publication No.: US08895244B2Publication Date: 2014-11-25
- Inventor: Akimitsu Okamoto , Kaori Sugizaki , Akiko Nakamura , Hiroyuki Yanagisawa , Shuji Ikeda
- Applicant: Akimitsu Okamoto , Kaori Sugizaki , Akiko Nakamura , Hiroyuki Yanagisawa , Shuji Ikeda
- Applicant Address: JP Wako-shi, Saitama
- Assignee: Riken
- Current Assignee: Riken
- Current Assignee Address: JP Wako-shi, Saitama
- Agency: Buchanan, Ingersoll & Rooney PC
- Priority: JP2011-090971 20110415
- International Application: PCT/JP2012/060265 WO 20120416
- International Announcement: WO2012/141324 WO 20121018
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; B01L3/00
Abstract:
Provided are a method and a kit for detecting 5-hydroxymethylcytosine in a nucleic acid. The method is a method for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising the steps of: (1) oxidizing 5-hydroxymethylcytosine in a nucleic acid sample by treating the nucleic acid sample with a tungstic acid-based oxidizing agent comprising peroxotungstic acid, tungstic acid, a salt thereof, or a combination thereof with a reoxidizing agent; and (2) determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample.
Public/Granted literature
- US20140045183A1 METHOD AND KIT FOR DETECTING 5-HYDROXYMETHYLCYTOSINE IN NUCLEIC ACIDS Public/Granted day:2014-02-13
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