发明授权
US08426132B2 Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
有权
用标记的探针和3'至5'核酸外切酶活性进行定量扩增
- 专利标题: Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
- 专利标题(中): 用标记的探针和3'至5'核酸外切酶活性进行定量扩增
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申请号: US12264200申请日: 2008-11-03
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公开(公告)号: US08426132B2公开(公告)日: 2013-04-23
- 发明人: Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
- 申请人: Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
- 申请人地址: US CA Hercules
- 专利权人: Bio-Rad Laboratories, Inc.
- 当前专利权人: Bio-Rad Laboratories, Inc.
- 当前专利权人地址: US CA Hercules
- 代理机构: Kilpatrick Townsend & Stockton LLP
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34
摘要:
This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
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