A test mixture for detecting the presence or absence of pathogenic staphylococci (“p.staphylococci”) directly in a first generation specimen test sample, the test mixture comprising: an effective amount of amino acids, an effective amount of nitrogen sources, an effective amount of salts, an effective amount of vitamins, an effective amount of calcium, and an effective amount of coagulase substrates, which coagulase substrates include fibrinogen, which will create a clot within a liquid medium containing the test mixture and the first generational specimen test sample at temperatures in the range of about 20° C. to about 42° C. in the presence of p.staphylococci in the first generation specimen test sample.