发明授权
- 专利标题: Method of forming planar lipid double membrane for membrane protein analysis and apparatus therefor
- 专利标题(中): 形成用于膜蛋白分析的平面脂质双膜的方法及其装置
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申请号: US10586331申请日: 2005-01-19
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公开(公告)号: US08039247B2公开(公告)日: 2011-10-18
- 发明人: Shoji Takeuchi , Hiroaki Suzuki , Hiroyuki Noji
- 申请人: Shoji Takeuchi , Hiroaki Suzuki , Hiroyuki Noji
- 申请人地址: JP Kawaguchi-shi
- 专利权人: Japan Science and Technology Agency
- 当前专利权人: Japan Science and Technology Agency
- 当前专利权人地址: JP Kawaguchi-shi
- 代理机构: Oblon, Spivak, McClelland, Maier & Neustadt, L.L.P.
- 优先权: JP2004-012995 20040121
- 国际申请: PCT/JP2005/000558 WO 20050119
- 国际公布: WO2005/071405 WO 20050804
- 主分类号: C12M1/34
- IPC分类号: C12M1/34 ; G01N1/28
摘要:
To provide a method of forming a planar lipid-bilayer membrane for membrane protein analysis, by which downsizing, simplifying, and multichanneling of a device therefore are achieved. A planar lipid-bilayer membrane 24 is formed by filling a microchannel 12 with a buffer solution 18, the microchannel 12 disposed under a horizontal partition wall 13 having an aperture 14; applying a small amount of a lipid solution 20 as a droplet on the aperture 14 filled with the buffer solution 18 to thereby form a thin layer 21 of the lipid solution in a chamber, the chamber 17 being formed at a position corresponding to the aperture 14 and provided with a liquid trap 15 inside the chamber; and applying a buffer solution 23 as a droplet to the chamber 17 from the upper side of the chamber.
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