Disclosed is a chemical reaction cartridge including a substrate which is a rigid body and an elastic body, and a flow path and two or more chambers connected by the flow path are formed inside the cartridge, the cartridge is structured so as to move or block a fluid substance in the flow path or the chamber by partially sealing the flow path, the chamber or the both of the flow path and the chamber by applying external force to the elastic body from outside, and the substrate includes convex portions which protrude further than the elastic body at a surface of the substrate, on which the elastic body is formed.