发明授权
- 专利标题： Method and device for improving protein stability and solubility
- 专利标题（中）： 提高蛋白质稳定性和溶解度的方法和装置
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申请号： US09848780申请日： 2001-05-03
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公开(公告)号： US06861403B2公开(公告)日： 2005-03-01
- 发明人: Mitchell C. Sanders
- 申请人： Mitchell C. Sanders
- 申请人地址： US MA Worcester
- 专利权人： Expressive Constructs, Inc.
- 当前专利权人： Expressive Constructs, Inc.
- 当前专利权人地址： US MA Worcester
- 代理机构： Hamilton, Brook, Smith & Reynolds, P.C.
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C07K1/13 ; C07K1/16 ; C07K14/435 ; C07K14/47 ; C12N15/70 ; C12P21/02 ; A61K38/00 ; C07K1/19
摘要：
A method for expressing proteins as a fusion chimera with a domain of p26 or alpha crystallin type proteins to improve the protein stability and solubility when over expressed in bacteria such as E. coli is provided. Genes of interest are cloned into the multiple cloning site of the Vector System just downstream of the p26 or alpha crystallin type protein and a thrombin cleavage site. Protein expression is driven by a strong bacterial promoter (TAC). The expression is induced by the addition of 1 mM IPTG that overcomes the lac repression (lac Iq). The soluble recombinant protein is purified using a fusion tag.
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