Invention Grant
- Patent Title: Fluid delivery system for capillary electrophoretic applications
- Patent Title (中): 用于毛细管电泳应用的流体输送系统
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Application No.: US09373909Application Date: 1999-08-13
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Publication No.: US06375819B1Publication Date: 2002-04-23
- Inventor: Qingbo Li , Changsheng Liu , Thomas E. Kane , John R. Kernan , Bernard Sonnenschein , Michael V. Sharer
- Applicant: Qingbo Li , Changsheng Liu , Thomas E. Kane , John R. Kernan , Bernard Sonnenschein , Michael V. Sharer
- Main IPC: G01N2726
- IPC: G01N2726

Abstract:
An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
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