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US5830726A Method for obtaining a modified heparinase gene 失效
获得修饰的肝素酶基因的方法

Method for obtaining a modified heparinase gene
摘要:
The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.
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