发明授权
- 专利标题: Method for obtaining a modified heparinase gene
- 专利标题(中): 获得修饰的肝素酶基因的方法
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申请号: US445342申请日: 1995-05-19
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公开(公告)号: US5830726A公开(公告)日: 1998-11-03
- 发明人: Ramnath Sasisekharan , Kelley Moremen , Charles I. Cooney , Joseph J. Zimmermann , Robert S. Langer
- 申请人: Ramnath Sasisekharan , Kelley Moremen , Charles I. Cooney , Joseph J. Zimmermann , Robert S. Langer
- 申请人地址: MA Cambridge
- 专利权人: Massachusetts Institute of Technology
- 当前专利权人: Massachusetts Institute of Technology
- 当前专利权人地址: MA Cambridge
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12N1/21 ; C12N9/88 ; C12N15/00 ; C12N15/60 ; C12R1/01 ; C12R1/20 ; C12N15/10
摘要:
The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.
公开/授权文献
- USD317044S Container for an air freshener 公开/授权日:1991-05-21
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