Invention Grant
- Patent Title: In situ recombinant PCR within single cells
- Patent Title (中): 单细胞内原位重组PCR
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Application No.: US190199Application Date: 1994-07-13
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Publication No.: US5830663APublication Date: 1998-11-03
- Inventor: Michael J. Embleton , Guy Gorochov , Peter T. Jones , Gregory P. Winter
- Applicant: Michael J. Embleton , Guy Gorochov , Peter T. Jones , Gregory P. Winter
- Applicant Address: GB2
- Assignee: Medical Research Council
- Current Assignee: Medical Research Council
- Current Assignee Address: GB2
- Priority: GBX9117352 19910810; GBX9212419 19920611
- Main IPC: C07K14/705
- IPC: C07K14/705 ; C07K14/00 ; C07K16/00 ; C07K19/00 ; C12N15/09 ; C12N15/10 ; C12N15/13 ; C12P21/08 ; C12Q1/68 ; C07H21/02 ; C12P19/34
Abstract:
Disclosed is a method of treating a heterogeneous population of cells to link together copies of two or more nucleic acid sequences from at least some of the cells, the arrangement being such that copies of the DNA sequences from an individual cell are preferentially linked in the vicinity of the nucleic acid from which the copies are derived. Also disclosed are recombinant proteins expressed by the method of the invention and kits for performing said method.
Public/Granted literature
- US4855587A X-ray image intensifier tube with carbon-reinforced plastic foil entrance window Public/Granted day:1989-08-08
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