Adjacent shaped grooves are placed in single crystal structure with great accuracy and known dimensions by a combination of anisotropic and isotropic etching to produce a scanning probe microscope calibration standard with fine V-shaped grooves forming a prismatically shaped ridge or blade between them. A probe microscope to be calibrated is used to profile the tip of the ridge in a number of places along the length of the ridge. With knowledge of the sidewall angles and tip radius of the calibration standard both the flat tip dimensions of a probe with a flared tip and the tip radius of a probe with a conical tip can be calculated from the profile they produce.