Invention Grant
- Patent Title: Method for the fluorimetric determination of endotoxins, new peptides carrying a fluorophorous substance usable in said method and method for its preparation
- Patent Title (中): 内毒素荧光测定方法，可用于所述方法的携带荧光物质的新肽及其制备方法
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Application No.: US422976Application Date: 1982-09-09
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Publication No.: US4576745APublication Date: 1986-03-18
- Inventor: Michel Monsigny
- Applicant: Michel Monsigny
- Applicant Address: FRX Paris
- Assignee: Centre National de la Recherche Scientifique
- Current Assignee: Centre National de la Recherche Scientifique
- Current Assignee Address: FRX Paris
- Priority: FRX8100261 19810109
- Main IPC: C07K1/113
- IPC: C07K1/113 ; C07K5/083 ; C07K5/103 ; C07K14/00 ; C07K14/195 ; C07K14/41 ; C12Q1/34 ; G01N33/52 ; G01N33/579 ; F25B29/00
Abstract:
Method for determining endotoxins in a liquid, wherein said liquid is contacted, at 20.degree.-40.degree. C., with a lysate of limule amebocytes and an aqueous solution of a peptide having the following formula: ##STR1## in which [Pept-- is a peptidic hydrophobic chain, n is 1, 2, 3, 4, or 5, B is ##STR2## being H or CH.sub.3, --NH--Ar] is the residue of a fluorescent heteroaromatic amine and X.sup..crclbar. is an anion, the pH of the thus obtained medium is adjusted at 5 to 10 and the heteroaromatic amine thus formed is determined by fluorimetry, and new peptides according to formula (I) usable in said method.
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