A method for producing 225Ac solution includes steps (I) to (III): a step (I) of passing a solution containing 226Ra and 225Ac through a solid-phase extraction agent (a) that contains a compound represented by formula (A) so as to cause the solid-phase extraction agent (a) to retain 225Ac; a step (II) of passing a liquid containing an eluate, which is obtained by eluting the retained 225Ac from the solid-phase extraction agent (a), through a solid-phase extraction agent (b) that contains a compound represented by formula (B) so as to cause the solid-phase extraction agent (b) to retain 225Ac; and a step (III) of eluting the retained 225Ac from the solid-phase extraction agent (b) to obtain an 225Ac solution.