A method for automatic glue-spraying of stringers and inspection of glue-spraying quality based on measured data. Three-dimensional (3D) point cloud data of a stringer-skin assembly is collected by 3D laser scanner, and then processed by denoising and sampling. Feature points of an intersection line of a site to be glued of the stringer-skin assembly are extracted by K-means clustering method based on Gaussian mapping, and a minimum spanning tree is constructed based on a set of the extracted feature points. A connected region is established to obtain an initial feature intersection line of the string-skin assembly, which is optimized by random sample consensus algorithm to remove redundant small branch structures to obtain the actual glue-spraying trajectory. The quality of the glue sprayed on the stringer-skin assembly is inspected by line laser to determine positions of the defects, which are then subjected to secondary glue-spraying.