A frame sealant and a method of preparing the same as well as a display device containing the frame sealant. The frame sealant includes an epoxy-acrylic resin, an acrylic resin, a thermal curing agent, a coupling agent, a photoinitiator, and an organic filler, and further an one-dimensional nano-material as an inorganic filler which is capable of cross-linking with the epoxy-acrylic resin. The one-dimensional nano-material after cross-linking with the epoxy-acrylic resin will form an interlaced network, which can prevent granular materials from precipitating and improve the display quality.