A preparation method of a glass film, a photoelectric device and a packaging method thereof, and a display device are provided, and the preparation method of a glass film includes: forming a sacrificial layer on a base substrate; forming a glass frit film on the sacrificial layer; solidifying the glass frit film; and removing the sacrificial layer, so as to obtain a glass film. The method can bring an individual glass film, which is helpful to a narrow-bezel design of a photoelectric device.