Invention Application
US20160010078A1 Two-Dimensional Cell Array Device and Apparatus for Gene Quantification and Sequence Analysis 审中-公开
用于基因定量和序列分析的二维细胞阵列装置和装置

  • Patent Title: Two-Dimensional Cell Array Device and Apparatus for Gene Quantification and Sequence Analysis
  • Patent Title (中): 用于基因定量和序列分析的二维细胞阵列装置和装置
  • Application No.: US14771339
    Application Date: 2013-03-12
  • Publication No.: US20160010078A1
    Publication Date: 2016-01-14
  • Inventor: Masataka SHIRAIHideki KAMBARAKiyomi TANIGUCHIMaiko TANABE
  • Applicant: HITACHI, LTD.
  • International Application: PCT/JP2013/056818 WO 20130312
  • Main IPC: C12N15/10
  • IPC: C12N15/10
Two-Dimensional Cell Array Device and Apparatus for Gene Quantification and Sequence Analysis
Abstract:
In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.
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