The present invention provides a fluorescence detection and photodynamic therapy apparatus including: a combined light source unit 10 including a plurality of coherent and non-coherent light sources 11, 12 and 13 configured to irradiate light onto a to-be-observed object while performing continuous illumination; an optical imaging unit 20 configured to form an image of the to-be-observed object 70 and project the image to an image processing/controlling system 34; a multispectral imaging unit 30 including a one-chip multispectral sensor and the image processing/controlling system 34; a blocking filter 40 installed between the to-be-observed object 70 and the one-chip multispectral sensor 32, the blocking filter being configured to block some light reflected off from the to-be-observed object 70 while allowing some light and fluorescent light to pass therethrough; a computer system 50 configured to process, analyze, reproduce and store the image acquired from the multispectral imaging unit 30, and transfer the image to a display device 60 and control the overall operation of all the related elements; and the display device 60 configured to display a processing result of the image by the computer system 50.