A headspace sample introduction device which collects a sample gas from a sample container and introduces the gas into a predetermined analyzer is provided. The device includes a sample tray 10 capable of holding at least one sample container 100 storing a liquid sample or a solid sample, a heating means 30 for heating at least one sample container 100 taken from the sample tray 10, a sample-gas collecting means 40 for collecting a sample gas from the upper space in the sample container 100 held in the heating means 30, and a cooling transfer means 20 for keeping and cooling at least one sample container 100 taken from the heating means 30 and returning the cooled sample container 100 to the sample tray 20. Since the headspace sample introduction device can return the heated sample containers 100 to the sample tray 10 after they are cooled with the cooling transfer means 20, the hot sample containers can be assuredly prevented from contacting the user.