• 专利标题: Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens
  • 申请号: US12930776
    申请日: 2011-02-07
  • 公开(公告)号: US20110171625A1
    公开(公告)日: 2011-07-14
  • 发明人: Michael D. Cecchi
  • 申请人: Michael D. Cecchi
  • 主分类号: A01N1/00
  • IPC分类号: A01N1/00
Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens
摘要:
A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the un-frozen specimens the same as the frozen specimens when recovering them from cryopreservation.
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