A gravure printing method is provided that includes: patterning a substrate to form a printing substrate having a recess portion and a convex portion; forming a first self-assembled monolayer on the surface of the recess portion; forming a second self-assembled monolayer on the surface of the convex portion; filling ink into the recess portion; and transferring the ink filled in the recess portion to a printing object substrate, wherein the first and second self-assembled monolayers respectively formed on the surfaces of the recess portion and the convex portion have different surface energies.