A body fluid sampling system is provided for use on a tissue site. A drive force generator is provided. A housing has at least a first handle portion with a first average diameter, and a head portion with a second average diameter that is larger than the first average diameter. The housing includes a plurality of analyte sensors and a plurality of sample chambers. Each analyte sensor is associated with a sample chamber and is configured to receive body fluid from a wound in tissue created by a penetrating member. A plurality of penetrating members are operatively coupled to the drive force generator. The drive force generator moves each of the penetrating members along a path out of the housing into the tissue site, stops in the tissue site, and withdraws out of the tissue site. Each penetrating member is at least partially positioned adjacent to an analyte sensor in a sample chamber. A user interface is included.