发明申请
US20070128629A1 Class I sequence based typing of HLA-A, -B, and -C alleles by direct DNA sequencing
审中-公开
通过直接DNA测序对HLA-A，-B和-C等位基因进行I类序列分型
- 专利标题： Class I sequence based typing of HLA-A, -B, and -C alleles by direct DNA sequencing
- 专利标题（中）： 通过直接DNA测序对HLA-A，-B和-C等位基因进行I类序列分型
-
申请号： US11524092申请日： 2006-09-20
-
公开(公告)号： US20070128629A1公开(公告)日： 2007-06-07
- 发明人: William Hildebrand , Mary Ellexson , Pierre Chretlen , R. Duthie
- 申请人： William Hildebrand , Mary Ellexson , Pierre Chretlen , R. Duthie
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
There is provided a method for directly typing or sequencing HLA-A, -B, or -C alleles from a tissue sample wherein exons 2 and 3 of the HLA-A, -B, or -C alleles from the sample are amplified together in a locus specific manner and then separated out and individually amplified in a locus specific manner. After the two amplifications, the amplified exons are directly sequenced, the sequences are recombined, and a comparison is made between the derived HLA allele sequence and an HLA allele database, thereby giving an exact HLA-A, -B, or -C type for the sample being tested.
信息查询
