The present invention disclosed a hanging disposable gloves cartridge, which includes a plurality of disposable gloves alignedly overlapped with each other to form a glove stack, wherein each of the disposable gloves has a perforated tear line transversely spanned thereon for separate a hand portion from a waist portion; a clamp holder, which includes a pair of mounting wings for substantially sandwiching the glove stack there between at a position above the tear lines of the disposable gloves, wherein each of the disposable gloves are made of two layers of film forming a hand cavity therebetween, a human hand is able to insert into the hand cavity to shell off an outermost dispose glove from the cartridge.